File:PCR simple.png
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Summary
PCR. Double stranded DNA is heated to approximately 95°C to break the two chains apart (denature). Then it is cooled to a temperature where short pieces of single stranded DNA (primers) can bind (anneal). These primers will be complementary to the sequences in the DNA template. The DNA is then heated to an intermediate temperature to allow the polymerase to build the DNA chain from the primer (extension). These three process are repeated to produce large amounts of new double stranded DNA.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 18:33, 3 January 2017 | | 1,280 × 1,707 (14 KB) | 127.0.0.1 (talk) | PCR. Double stranded DNA is heated to approximately 95°C to break the two chains apart (denature). Then it is cooled to a temperature where short pieces of single stranded DNA (primers) can bind (anneal). These primers will be complementary to the sequences in the DNA template. The DNA is then heated to an intermediate temperature to allow the polymerase to build the DNA chain from the primer (extension). These three process are repeated to produce large amounts of new double stranded DNA. |
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