Ribonuclease L

Ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
250px PDB rendering based on 1wdy.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1WDY, 4G8K, 4G8L, 4OAU, 4OAV
Identifiers
Symbols	RNASEL ; PRCA1; RNS4
External IDs	OMIM: 180435 MGI: 1098272 HomoloGene: 8040 ChEMBL: 3575 GeneCards: RNASEL Gene
EC number	3.1.26.-, 3.1.27.-
Gene ontology Molecular function • RNA binding • endoribonuclease activity • protein kinase activity • protein binding • ATP binding • rRNA binding • ribonucleoprotein complex binding • metal ion binding Cellular component • cellular_component • mitochondrial matrix • cytosol • nuclear matrix Biological process • rRNA processing • mRNA processing • protein phosphorylation • cytokine-mediated signaling pathway • regulation of mRNA stability • negative regulation of viral genome replication • fat cell differentiation • positive regulation of transcription from RNA polymerase II promoter • defense response to virus • transmembrane transport • type I interferon signaling pathway • RNA phosphodiester bond hydrolysis, endonucleolytic • positive regulation of glucose import in response to insulin stimulus Sources: Amigo / QuickGO
RNA expression pattern
File:PBB GE RNASEL 221287 at tn.png
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	6041	24014
Ensembl	ENSG00000135828	ENSMUSG00000066800
UniProt	Q05823	Q05921
RefSeq (mRNA)	NM_021133	NM_011882
RefSeq (protein)	NP_066956	NP_036012
Location (UCSC)	Chr 1: 182.57 – 182.59 Mb	Chr 1: 153.75 – 153.76 Mb
PubMed search	[1]	[2]

Ribonuclease L or RNase L (for latent), known sometimes as ribonuclease 4 or 2'-5' oligoadenylate synthetase-dependent ribonuclease — is an interferon (IFN)-induced ribonuclease which, upon activation, destroys all RNA within the cell (both cellular and viral). RNase L is an enzyme that in humans is encoded by the RNASEL gene.

This gene encodes a component of the interferon-regulated 2'-5'oligoadenylate (2'-5'A) system that functions in the antiviral and antiproliferative roles of interferons. RNase L is activated by dimerization, which occurs upon 2'-5'A binding, and results in cleavage of all RNA in the cell. This can lead to activation of MDA5, an RNA helicase involved in the production of interferons.

Synthesis and activation

File:RNAse L Pathway new.png

RNase L activation pathway-IFN factors bind the receptor and lead transcription and modifications of OAS. Viral dsRNA binds OAS, so that 2'-5'A is produced leading to the dimerization of RNase L. Activated RNase L cleaves all RNA in the cell, which can activate MDA5 leading to interferon production.

RNase L is present in very minute quantities during the normal cell cycle. When interferon binds to cell receptors, it activates transcription of around 300 genes to bring about the antiviral state. Among the enzymes produced is RNase L, which is initially in an inactive form. A set of transcribed genes codes for 2’-5’ Oligoadenylate Synthetase (OAS). The transcribed RNA is then spliced and modified in the nucleus before reaching the cytoplasm and being translated into an inactive form of OAS. The location of OAS in the cell and the length of the 2’-5’ oligoadenylate depends on the post-transcriptional and post-translational modifications of OAS.

OAS is only activated under a viral infection, when a tight binding of the inactive form of the protein with a viral dsRNA, consisting of the retrovirus’ ssRNA and its complementary strand, takes place. Once active, OAS converts ATP to pyrophosphate and 2’-5’-linked oligoadenylates (2-5A), which are 5’ end phosphorylated. 2-5 A molecules then bind to RNase L, promoting its activation by dimerization. In its activated form RNase L cleaves all RNA molecules in the cell leading to autophagy and apoptosis. Some of the resulting RNA fragments can also further induce the production of INF-β as noted in the Significance section.

This dimerization and activation of RNase L can be recognized using Fluorescence Resonance Energy Transfer (FRET), as oligoribonucleotides containing a quencher and a fluorophore on opposite sites are added to a solution with inactive RNase L. The FRET signal is then recorded as the quencher and the fluorophore are very close to each other. Upon the addition of 2-5A molecules, RNase L becomes active, cleaving the oligoribonucleotides and interfering in the FRET signal.

Significance

RNase L is part of the body's innate immune defense, namely the antiviral state of the cell. When a cell is in the antiviral state, it is highly resistant to viral attacks and is also ready to undergo apoptosis upon successful viral infection. Degradation of all RNA within the cell (which usually occurs with cessation of translation activity caused by protein kinase R) is the cell's last stand against a virus before it attempts apoptosis.

Interferon beta (INF-β), a type I interferon responsible for antiviral activity, is produced by RNAse L and melanoma differentiation-associated protein 5 (MDA5) in the infected cell. The relationship between RNAse L and MDA5 in the production of INFs has been confirmed with siRNA tests silencing the expression of either molecule and noting a marked decline in INF production. MDA5, an RNA helicase, is known to be activated by long dsRNA transcribed from the viral genome. In a cell with RNAse L, MDA5 activity may be further enhanced. When active, RNAse L cleaves and identifies viral RNA and feeds it into MDA5 activation sites, enhancing the production of INF-β. The RNA fragments produced by RNAse L have double stranded regions, as well as specific markers, that allow them to be identified by the RNAse L and MDA5. Some studies have suggested that high levels of RNAse L may actually inhibit INF-β production, but a clear linkage still exists between RNAse L activity and INF-β production.

Furthermore, it has been shown that RNase L is involved in many diseases. In 2002, the “hereditary prostate cancer 1” locus (HPC1) was mapped to the RNASEL gene, indicating that mutations in this gene cause a predisposition to prostate cancer. Impairments of the OAS/RNase L pathway in chronic fatigue syndrome (CFS) have been investigated.

External links

• ribonuclease L, human at the US National Library of Medicine Medical Subject Headings (MeSH)

Category:Ribonucleases Category:Prostate cancer