Adiponectin

Adiponectin, C1Q and collagen domain containing
PDB rendering based on 1c28.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 4DOU
Identifiers
Symbols	ADIPOQ ; ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28
External IDs	OMIM: 605441 MGI: 106675 HomoloGene: 3525 GeneCards: ADIPOQ Gene
Gene ontology Molecular function • receptor binding • cytokine activity • hormone activity • protein binding • sialic acid binding • identical protein binding • protein homodimerization activity Cellular component • extracellular region • collagen trimer • extracellular space • endoplasmic reticulum • cell surface • extracellular exosome Biological process • response to hypoxia • positive regulation of protein phosphorylation • glucose metabolic process • generation of precursor metabolites and energy • fatty acid beta-oxidation • response to nutrient • circadian rhythm • response to sucrose • response to glucose • positive regulation of signal transduction • negative regulation of platelet-derived growth factor receptor signaling pathway • positive regulation of protein kinase A signaling • negative regulation of macrophage derived foam cell differentiation • negative regulation of tumor necrosis factor-mediated signaling pathway • positive regulation of cholesterol efflux • regulation of glucose metabolic process • response to activity • negative regulation of smooth muscle cell migration • fatty acid oxidation • negative regulation of cell migration • negative regulation of granulocyte differentiation • negative regulation of protein autophosphorylation • positive regulation of cellular protein metabolic process • negative regulation of tumor necrosis factor production • positive regulation of interleukin-8 production • cellular response to insulin stimulus • positive regulation of myeloid cell apoptotic process • adiponectin-activated signaling pathway • negative regulation of heterotypic cell-cell adhesion • low-density lipoprotein particle clearance • response to tumor necrosis factor • cellular response to drug • glucose homeostasis • positive regulation of I-kappaB kinase/NF-kappaB signaling • negative regulation of I-kappaB kinase/NF-kappaB signaling • negative regulation of MAP kinase activity • response to ethanol • negative regulation of fat cell differentiation • negative regulation of macrophage differentiation • negative regulation of low-density lipoprotein particle receptor biosynthetic process • negative regulation of gluconeogenesis • negative regulation of blood pressure • positive regulation of blood pressure • negative regulation of transcription, DNA-templated • positive regulation of fatty acid metabolic process • positive regulation of glucose import • negative regulation of hormone secretion • negative regulation of smooth muscle cell proliferation • negative regulation of inflammatory response • positive regulation of peptidyl-tyrosine phosphorylation • negative regulation of phagocytosis • negative regulation of synaptic transmission • brown fat cell differentiation • protein homooligomerization • response to glucocorticoid • membrane depolarization • membrane hyperpolarization • protein heterotrimerization • negative regulation of ERK1 and ERK2 cascade • response to linoleic acid • detection of oxidative stress • cellular response to cAMP • positive regulation of monocyte chemotactic protein-1 production • cellular response to epinephrine stimulus • protein localization to plasma membrane • negative regulation of intracellular protein transport • negative regulation of receptor binding • negative regulation of DNA biosynthetic process • positive regulation of glycogen (starch) synthase activity • positive regulation of metanephric glomerular visceral epithelial cell development • positive regulation of cAMP-dependent protein kinase activity • positive regulation of renal albumin absorption • negative regulation of platelet-derived growth factor receptor-alpha signaling pathway • negative regulation of metanephric mesenchymal cell migration Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9370	11450
Ensembl	ENSG00000181092	ENSMUSG00000022878
UniProt	Q15848	Q60994
RefSeq (mRNA)	NM_001177800	NM_009605
RefSeq (protein)	NP_001171271	NP_033735
Location (UCSC)	Chr 3: 186.84 – 186.86 Mb	Chr 16: 23.15 – 23.16 Mb
PubMed search	[1]	[2]

Adiponectin (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is a protein which in humans is encoded by the ADIPOQ gene. It is involved in regulating glucose levels as well as fatty acid breakdown.

Structure

Adiponectin is a 244-amino-acid-long polypeptide (protein). There are four distinct regions of adiponectin. The first is a short signal sequence that targets the hormone for secretion outside the cell; next is a short region that varies between species; the third is a 65-amino acid region with similarity to collagenous proteins; the last is a globular domain. Overall this gene shows similarity to the complement 1Q factors (C1Q). However, when the 3-dimensional structure of the globular region was determined, a striking similarity to TNFα was observed, despite unrelated protein sequences.

Function

Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. Adiponectin is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones. Levels of the hormone are inversely correlated with body fat percentage in adults; however, a meta analysis was not able to confirm this association in healthy adults. The association in infants and young children is less clear. Similarly, circulating adiponectin concentrations increase during caloric restriction in animals and humans, such as in patients with anorexia nervosa. This observation is surprising, given that adiponectin is produced by adipose tissue; however, a recent study suggests that adipose tissue within bone marrow, which increases during caloric restriction, contributes to elevated circulating adiponectin in this context.

Transgenic mice with increased adiponectin show impaired adipocyte differentiation and increased energy expenditure associated with protein uncoupling. The hormone plays a role in the suppression of the metabolic derangements that may result in type 2 diabetes, obesity, atherosclerosis, non-alcoholic fatty liver disease (NAFLD) and an independent risk factor for metabolic syndrome. Adiponectin in combination with leptin has been shown to completely reverse insulin resistance in mice.

Adiponectin is secreted into the bloodstream where it accounts for approximately 0.01% of all plasma protein at around 5-10 μg/mL. Plasma concentrations reveal a sexual dimorphism, with females having higher levels than males. Levels of adiponectin are reduced in diabetics compared to non-diabetics. Weight reduction significantly increases circulating levels.

Adiponectin automatically self-associates into larger structures. Initially, three adiponectin molecules bind together to form a homotrimer. The trimers continue to self-associate and form hexamers or dodecamers. Like the plasma concentration, the relative levels of the higher-order structures are sexually dimorphic, where females have increased proportions of the high-molecular weight forms. Recent studies showed that the high-molecular weight form may be the most biologically active form regarding glucose homeostasis. High-molecular-weight adiponectin was further found to be associated with a lower risk of diabetes with similar magnitude of association as total adiponectin. However, coronary artery disease has been found to be positively associated with high molecular weight adiponectin, but not with low molecular weight adiponectin.

Adiponectin exerts some of its weight reduction effects via the brain. This is similar to the action of leptin, but the two hormones perform complementary actions, and can have synergistic effects.

Receptors

Main article: Adiponectin receptor

Adiponectin binds to a number of receptors. So far, two receptors have been identified with homology to G protein-coupled receptors, and one receptor similar to the cadherin family:

• Adiponectin receptor 1 (AdipoR1)
• Adiponectin receptor 2 (AdipoR2)
• T-cadherin - CDH13

These have distinct tissue specificities within the body and have different affinities to the various forms of adiponectin. The receptors affect the downstream target AMP kinase, an important cellular metabolic rate control point. Expression of the receptors is correlated with insulin levels, as well as reduced in mouse models of diabetes, particularly in skeletal muscle and adipose tissue.

Discovery

Adiponectin was first characterised in 1995 in differentiating 3T3-L1 adipocytes (Scherer PE et al). In 1996 it was characterised in mice as the mRNA transcript most highly expressed in adipocytes (Maeda, 1996 (citation #1, below)). In 2007, adiponectin was identified as a transcript highly expressed in preadipocytes (precursors of fat cells) differentiating into adipocytes.

The human homologue was identified as the most abundant transcript in adipose tissue. Contrary to expectations, despite being produced in adipose tissue, adiponectin was found to be decreased in obesity. This downregulation has not been fully explained. The gene was localised to chromosome 3q27, a region highlighted as affecting genetic susceptibility to type 2 diabetes and obesity. Supplementation by differing forms of adiponectin was able to improve insulin control, blood glucose and triglyceride levels in mouse models.

The gene was investigated for variants that predispose to type 2 diabetes. Several single nucleotide polymorphisms in the coding region and surrounding sequence were identified from several different populations, with varying prevalences, degrees of association and strength of effect on type 2 diabetes. Berberine, an herbal folk medicine, has been shown to increase adiponectin expression which partly explains its beneficial effects on metabolic disturbances. Mice fed the omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have shown increased plasma adiponectin. Curcumin, capsaicin, gingerol, and catechins have also been found to increase adiponectin expression.

Phylogenetic distribution includes expression in birds and fish.

Metabolic

Adiponectin effects:

• glucose flux
  • decreased gluconeogenesis
  • increased glucose uptake
• lipid catabolism
  • β-oxidation
  • triglyceride clearance
• protection from endothelial dysfunction (important facet of atherosclerotic formation)
• insulin sensitivity
• weight loss
• control of energy metabolism.
• upregulation of uncoupling proteins
• reduction of TNF-alpha

Regulation of adiponectin

• Obesity is associated with decreased adiponectin.
  • The exact mechanism of regulation is unknown, but adiponectin could be regulated by post-translational mechanisms in cells.

Hypoadiponectinemia

A low level of adiponectin is an independent risk factor for developing:

• Metabolic syndrome
• Diabetes mellitus

Other

Lower levels of adiponectin are associated with ADHD in adults.

Adiponectin levels were found to be increased in rheumatoid arthritis patients responding to DMARDs or TNF inhibitor therapy.

Exercise induced release of adiponectin increased hippocampal growth and led to antidepressive symptoms in mice.

As a drug target

Circulating levels of adiponectin can indirectly be increased through lifestyle modifications and certain drugs such as statins.

A small molecule adiponectin receptor AdipoR1 and AdipoR2 agonist, AdipoRon, has been reported.

Extracts of sweet potatoes have been reported to increase levels of adiponectin and thereby improve glycemic control in humans. However, a systematic review concluded there is insufficient evidence to support the consumption of sweet potatoes to treat type 2 diabetes mellitus.

Adiponectin is apparently able to cross the blood-brain-barrier. However, conflicting data on this issue exist. Adiponectin has a half-life of 2.5 hours in humans.