Alfred G. Gilman

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Alfred G. Gilman
Alfred Goodman Gilman (cropped).jpg
Born Alfred Goodman Gilman
July 1, 1941
New Haven, Connecticut
Died December 23, 2015(2015-12-23) (aged 74)
Dallas, Texas, U.S.
Nationality American
Fields Pharmacologist
Alma mater Yale University (B.S., 1962)
Case Western Reserve University (MD-Ph.D., 1969)
Known for G-proteins
Notable awards John J. Abel Award (1975)
Richard Lounsbery Award (1987)
Louisa Gross Horwitz Prize (1989)
Nobel Prize in Physiology or Medicine (1994)
Spouse Kathryn Hedlund
Children 3

Alfred Goodman Gilman (July 1, 1941 – December 23, 2015) was an American pharmacologist and biochemist. He shared the 1994 Nobel Prize in Physiology or Medicine with Martin Rodbell for their discoveries regarding G-proteins.

G-proteins are a vital intermediary between the extracellular activation of receptors (GPCR) on the cell membrane and actions within the cell. Rodbell had shown in the 1960s that GTP was involved in cell signaling. It was Gilman who actually discovered the proteins that interacted with the GTP to initiate signalling cascades within the cell.

Early life

Gilman was born in New Haven, Connecticut. His parents were Mabel (Schmidt) and Alfred Gilman, a professor at the Yale School of Medicine and one of the authors of the classic pharmacology textbook The Pharmacological Basis of Therapeutics; he chose his son's middle name in honor of his co-author Louis S. Goodman.[1] Gilman served as one of the textbook's editors from 1980 to 2000, first collaborating with, then succeeding his father and Goodman.[2]

Education

Gilman graduated from Yale with his B.S. in 1962. He then entered a combined MD-PhD program at Case Western Reserve University School of Medicine in Cleveland, Ohio where he wanted to study under Nobel laureate pharmacologist Earl Sutherland. Sutherland was departing for Vanderbilt University, so Gilman studied with Sutherland's collaborator, Theodore Rall. Gilman graduated from Case Western in 1969, then did his post-doctoral studies at the National Institutes of Health with Nobel laureate Marshall Nirenberg from 1969-71.

Career

In 1971, Gilman became a professor of pharmacology at the University of Virginia, School of Medicine, in Charlottesville, Virginia. In 1981, he became chairman of the Department of Pharmacology at the University of Texas Southwestern Medical Center at Dallas. He was elected as a member of the National Academy of Sciences in 1986.

He received the Albert Lasker Award for Basic Medical Research as well as the Louisa Gross Horwitz Prize from Columbia University in 1989 together with Edwin Krebs. In 2005, he was elected as Dean of University of Texas Southwestern Medical School, Dallas. He served on the board of advisors of Scientists and Engineers for America, an organization focused on promoting sound science in American government.

Death

Gilman died after a long battle with pancreatic cancer in Dallas, Texas on December 23, 2015 at the age of 74.[3]

Key papers

Norepinephrine stimulated increase of cyclic AMP levels in developing mouse brain cell cultures. Science. 1971 October 15;174(6): 292. PMID 4330303.

Regulation of adenosine 3',5'-cyclic monophosphate metabolism in cultured neuroblastoma cells. Nature. 1971 December 10;234(5328):356-58. PMID 4332686.

Fluorescent modification of adenosine 3',5'-monophosphate: spectroscopic properties and activity in enzyme systems. Science. 1972 July 21;177(45): 279-80. PMID 4339302.

The regulatory component of adenylate cyclase. Purification and properties. J Biol Chem. 1981 November 25; 256(22): 11517-526. PMID 6271754.

The regulatory component of adenylate cyclase. Purification and properties of the turkey erythrocyte protein. J Biol Chem. 1981 December 25;256(24): 12911-919. PMID 6273414.

Requirements for cholera toxin-dependent ADP-ribosylation of the purified regulatory component of adenylate cyclase. J Biol Chem. 1982 January 10;257(1): 20-3. PMID 6273425.

The guanine nucleotide activating site of the regulatory component of adenylate cyclase. Identification by ligand binding. J Biol Chem. 1982 October 10; 257(19): 11416-423. PMID 6288684.

The regulatory components of adenylate cyclase and transducin. A family of structurally homologous guanine nucleotide-binding proteins. J Biol Chem. 1983 June 10; 258(11): 7059-63. PMID 6304074.

The subunits of the stimulatory regulatory component of adenylate cyclase. Resolution, activity, and properties of the 35,000-dalton (beta) subunit. J Biol Chem. 1983 September 25; 258(18): 11361-368. PMID 6309843.

The subunits of the stimulatory regulatory component of adenylate cyclase. Resolution of the activated 45,000-dalton (alpha) subunit. J Biol Chem. 1983 September 25; 258(18): 11369-376. PMID 6309844.

Homologies between signal transducing G proteins and ras gene products. Science. 1984 November 16; 226(4676): 860-62. PMID 6436980.

G proteins and dual control of adenylate cyclase. Cell. 1984 Mar;36(3): 577-79. PMID 6321035.

Inhibition of receptor-mediated release of arachidonic acid by pertussis toxin. Cell. 1984 Dec;39(2 Pt 1): 301-08. PMID 6094010.

Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase. Science. 1985 September 20;229(4719): 1274-77. PMID 3839937.

Splice variants of the alpha subunit of the G protein Gs activate both adenylyl cyclase and calcium channels. Science. 1989 February 10;243(4892): 804-07. PMID 2536957.

Adenylyl cyclase amino acid sequence: possible channel- or transporter-like structure. Science. 1989 June 30;244(4912): 1558-64. PMID 2472670.

Type-specific regulation of adenylyl cyclase by G protein beta gamma subunits. Science. 1991 December 6; 254(5037): 1500-03. PMID 1962211.

Inhibition of adenylyl cyclase by Gi alpha. Science. 1993 July 9;261(5118): 218-21. PMID 8327893.

Recombinant G-protein beta gamma-subunits activate the muscarinic-gated atrial potassium channel. Nature. 1994 March 17; 368(6468): 255-57. PMID 8145826.

Structures of active conformations of Gi alpha 1 and the mechanism of GTP hydrolysis. Science. 1994 September 2;265(5177): 1405-12. doi:10.1126/science.8073283 PMID 8073283.

Construction of a soluble adenylyl cyclase activated by Gs alpha and forskolin. Science. 1995 June 23;268(5218):1769-72. PMID 7792604.

Tertiary and quaternary structural changes in Gi alpha 1 induced by GTP hydrolysis. Science. 1995 November 10;270(5238): 954-60. PMID 7481799.

The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2. Cell. 1995 December 15;83(6): 1047-58. PMID 8521505.

GAIP and RGS4 are GTPase-activating proteins for the Gi subfamily of G protein alpha subunits. Cell. 1996 August 9; 86(3): 445-52. doi:10.1016/S0092-8674(00)80117-8 PMID 8756726.

Crystal structure of the adenylyl cyclase activator Gsalpha. Science. 1997 December 12; 278(5345): 1943-47. PMID 9395396.

Crystal structure of the catalytic domains of adenylyl cyclase in a complex with Gsalpha.GTPgammaS. Science. 1997 December 12; 278(5345): 1907-16. PMID 9417641.

Further reading

See also

References

  1. Gilman, Alfred G. (1994). "Alfred G. Gilman - Biographical". Nobelprize.org. Retrieved March 25, 2015.<templatestyles src="Module:Citation/CS1/styles.css"></templatestyles>
  2. Dr. Alfred Gilman dies dies at 75, nytimes.com, January 14, 1985.
  3. "Dr. Alfred G. Gilman, Whose Work on Proteins Won Nobel Prize, Dies at 74". NewYorkTimes.com. Retrieved December 24, 2015.<templatestyles src="Module:Citation/CS1/styles.css"></templatestyles>

External links