Gamma-glutamyl transpeptidase

Not to be confused with GGGT.

Gamma-glutamyltranspeptidase
Identifiers
Symbol	G_glu_transpept
Pfam	PF01019
InterPro	IPR000101
PROSITE	PDOC00404
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

gamma-glutamyltransferase
Identifiers
EC number	2.3.2.2
CAS number	Template:CAS
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

gamma-glutamyltransferase 1
Identifiers
Symbol	GGT1
Alt. symbols	GGT
Entrez	2678
HUGO	4250
OMIM	231950
RefSeq	NM_001032364
UniProt	P19440
Other data
EC number	2.3.2.2
Locus	Chr. 22 q11.1-11.2

gamma-glutamyltransferase 2
Identifiers
Symbol	GGT2
Alt. symbols	GGT
Entrez	2679
HUGO	4251
OMIM	137181
RefSeq	NM_002058
UniProt	P36268
Other data
EC number	2.3.2.2
Locus	Chr. 22 q11.1-11.2

Gamma-glutamyl transferase or gamma-glutamyl transpeptidase (also γ-glutamyl transferase, GGT, GGTP, gamma-GT) (EC 2.3.2.2) is an enzyme that transfers gamma-glutamyl functional groups. It is found in many tissues, the most notable one being the liver, and has significance in medicine as a diagnostic marker.

GGT catalyzes the transfer of the gamma-glutamyl moiety of glutathione to an acceptor that may be an amino acid, a peptide or water (forming glutamate). GGT plays a key role in the gamma-glutamyl cycle, a pathway for the synthesis and degradation of glutathione and drug and xenobiotic detoxification. Other lines of evidence indicate that GGT can also exert a prooxidant role, with regulatory effects at various levels in cellular signal transduction and cellular pathophysiology.

Function

GGT is present in the cell membranes of many tissues, including the kidneys, bile duct, pancreas, gallbladder, spleen, heart, brain, and seminal vesicles. It is involved in the transfer of amino acids across the cellular membrane and leukotriene metabolism. It is also involved in glutathione metabolism by transferring the glutamyl moiety to a variety of acceptor molecules including water, certain L-amino acids, and peptides, leaving the cysteine product to preserve intracellular homeostasis of oxidative stress. This general reaction is:

(5-L-glutamyl)-peptide + an amino acid $\rightleftharpoons$ peptide + 5-L-glutamyl amino acid

Structural studies

In prokaryotes and eukaryotes, it is an enzyme that consists of two polypeptide chains, a heavy and a light subunit, processed from a single chain precursor by an autocatalytic cleavage. The active site of GGT is known to be located in the light subunit.

Medical applications

GGT is predominantly used as a diagnostic marker for liver disease in medicine, latent elevations in GGT are typically seen in patients with chronic viral hepatitis infections often taking 12 months or more to present.

Elevated serum GGT activity can be found in diseases of the liver, biliary system, and pancreas. In this respect, it is similar to alkaline phosphatase (ALP) in detecting disease of the biliary tract. Indeed, the two markers correlate well, though there is conflicting data about whether GGT has better sensitivity. In general, ALP is still the first test for biliary disease. The main value of GGT over ALP is in verifying that ALP elevations are, in fact, due to biliary disease; ALP can also be increased in certain bone diseases, but GGT is not. More recently, slightly elevated serum GGT has also been found to correlate with cardiovascular diseases and is under active investigation as a cardiovascular risk marker. GGT in fact accumulates in atherosclerotic plaques, suggesting a potential role in pathogenesis of cardiovascular diseases, and circulates in blood in the form of distinct protein aggregates, some of which appear to be related to specific pathologies such as metabolic syndrome, alcohol addiction and chronic liver disease. High body mass index is associated with type 2 diabetes only in persons with high serum GGT.

GGT is elevated by large quantities of alcohol ingestion. However, determination of high levels of total serum GGT activity is not specific to alcohol intoxication, and the measurement of selected serum forms of the enzyme offer more specific information. Isolated elevation or disproportionate elevation compared to other liver enzymes (such as ALP or ALT) can indicate alcohol abuse or alcoholic liver disease. and can indicate excess alcohol consumption up to 3 or 4 weeks prior to the test. The mechanism for this elevation is unclear. Alcohol might increase GGT production by inducing hepatic microsomal production, or it might cause the leakage of GGT from hepatocytes.

Numerous drugs can raise GGT levels, including barbiturates and phenytoin. GGT elevation has also been occasionally reported following NSAIDs, St. John's wort, Kava, and aspirin. Elevated levels of GGT can also be due to congestive heart failure.

Individual test results should always be interpreted using the reference range from the laboratory that performed the test though example reference ranges are 15-85 IU/L for men, and 5-55 IU/L for women.

Examples

Human proteins that belong to this family include:

GGT1, GGT2, GGT6, GGTL3, GGTL4, GGTLA1, GGTLA4

External links

• MedlinePlus Encyclopedia 003458
• gamma-Glutamyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)
• GGT - Lab Tests Online