Host–parasite coevolution

Host–parasite coevolution is a special case of coevolution, which is defined as the reciprocal adaptive genetic change of two antagonists (e.g. different species or genes) through reciprocal selective pressures. In the particular case of host–parasite coevolution the antagonists are different species of host and parasite.

Introduction

Hosts and parasites exert reciprocal selective pressures on each other, which may lead to rapid reciprocal adaptation. For organisms with short generation times host–parasite coevolution can be observed in comparatively small time periods, making it possible to study evolutionary change in real-time under both field and laboratory conditions. These interactions may thus serve as a counter-example to the common notion that evolution can only be detected across extended time.

The high dynamics associated with these interactions are summarized in the Red Queen hypothesis. It states that "it takes all the running you can do to keep in the same place", i.e. both host and parasite have to change continuously to keep up with each other's adaptations.

Relevance

Host-parasite coevolution is a ubiquitous phenomenon of potential importance to all living organisms, including humans. Many medically relevant diseases (e.g. malaria, AIDS and influenza) are caused by coevolving parasites. Therefore, detailed understanding of the coevolutionary adaptations between parasite ‘attack strategy’ and host immune system may result in the development of novel medications and vaccines and thus help save human life.

Furthermore, these interactions are of major concern in agriculture. Coevolving parasites are responsible for crop failure and especially in industrial agriculture, pesticides are indispensable. Livestock is similarly affected by numerous coevolving parasites. Therefore, a clearer understanding of host–parasite coevolution will point to new possibilities for organic farming and reduce the application of ecologically harmful chemicals.

Selection Dynamics

Host–parasite coevolution is characterized by reciprocal genetic change and thus changes in allele frequencies within populations. These changes may be determined by three main types of selection dynamics.

Negative frequency dependent selection

An allele is subject to negative frequency dependent selection if a rare allelic variant has a selective advantage. For example, the parasite should adapt to the most common host genotype, because it can then infect a large number of hosts. In turn, a rare host genotype may then be favored by selection, its frequency will increase and eventually it becomes common. Subsequently the parasite should adapt to the former infrequent genotype.

Coevolution determined by negative frequency dependent selection is rapid, potentially occurring across few generations. It maintains high genetic diversity by favoring uncommon alleles. This selection mode is expected for multicellular hosts, because adaptations can occur without the need for novel advantageous mutations, which are less likely to be frequent in these hosts because of relatively small population sizes and relatively long generation times.

Overdominant selection

Overdominance occurs if the heterozygote phenotype has a fitness advantage over both homozygotes ("heterozygote advantage" or "heterosis"). One example is sickle cell anemia. It is due to a mutation in the hemoglobin gene leading to sickle shape formation of red blood cells, causing clotting of blood vessels, restricted blood flow and reduced oxygen transport. At the same time, the mutation confers resistance to malaria, caused by Plasmodium parasites, which are passed off in red blood cells after transmission to humans by mosquitoes. Hence, homozygote and heterozygote genotypes for the sickle-cell disease allele show malaria resistance, while the homozygote suffers from severe disease phenotype. The alternative homozygote, which does not carry the sickle cell disease allele, is susceptible to Plasmodium. As a consequence, the heterozygote genotype is selectively favored in areas with a high incidence of malaria.

Directional selection

If an allele provides a fitness benefit, its frequency will increase within a population - selection is directional or positive. Selective sweeps are one form of directional selection, where the increase in frequency will eventually lead to the fixation of the advantageous allele. The process is considered to be slower in comparison to negative frequency dependent selection. It may produce an "arms race", consisting of the repeated origin and fixation of new parasite virulence and host defence traits.

This mode of selection is likely to occur in interactions between unicellular organisms and viruses due to large population sizes, short generation times, often haploid genomes and horizontal gene transfer, which increase the probability of beneficial mutations arising and spreading through populations.

Current challenges

The relative importance of these different selective dynamics for host–parasite coevolution is still under debate. One problem is that the unambiguous identification of the underlying dynamics is not straightforward. For example, if the plot of allele frequency changes indicates a selective sweep, then it is possible that the curve represents a small part of the fluctuating cycles expected for negative frequency dependent selection and that the time scale of the analysis is too short to allow clear discrimination of the alternatives.

In addition the modes of selection can differ between the various loci involved in host–parasite interactions. Therefore, the various loci may be subject to different selection dynamics.

Geographic Mosaic Theory of Coevolution

The Geographic Mosaic Theory of Coevolution by John N. Thompson hypothesizes that there is spatially divergent coevolutionary selection, producing genetic differentiation across populations.

The model assumes that there are three crucial elements that jointly fuel ongoing coevolutionary change:

1) There is a selection mosaic among populations. Natural selection on interspecific interactions differs among populations. Thus genotype-by-genotype-by-environment (G x G x E) interactions affect fitness of the antagonists. In other words, the specific environmental conditions determine how any genotype of one species influences the fitness of another species (biotic and abiotic components).

2) There are coevolutionary hotspots. Coevolutionary hotspots are communities in which selection on the interaction is truly reciprocal. These hotspots are intermixed with so-called coldspots in which only one or neither species adapts to the antagonist.

3) There is a geographic mixing of traits. Between the communities/regions there is a continuous "mixing" of traits by gene flow, random genetic drift, population extinction, or mutations. This remixing determines the exact dynamics of the Geographic Mosaic by shifting the spatial distributions of potentially coevolving alleles and traits.

In summary, the theory claims that species interactions commonly coevolve as complex geographic mosaics of populations, formed by differences in local selection and gene flow. It ties together the processes operating over space and time to determine the outcome of coevolutionary interactions and furthermore provides a reference framework for future (especially field) research on this topic.

Costs of adaptation

Resources are generally limited. Therefore, investment in one trait (e.g. virulence or immunity) limits investment in other life-history traits (e.g. reproductive rate). Moreoever, genes often have pleiotropic effects. Thus, a change in a pleiotropic immunity or virulence gene may automatically affect other traits. There is thus a trade-off between benefits and costs of the adaptive changes that then may prevent the host population to become fully resistant or the parasite population to express very high pathogenicity.

One example for costs related to gene pleiotropy was found for coevolving Escherichia coli and bacteriophages.

To inject their genetic material, phages need to bind to a specific bacterial cell surface receptor. The bacterium may prevent injection by altering the relevant binding site, e.g. in response to point mutations or deletion of the receptor. However, these receptors have important functions in bacterial metabolism. Their loss would thus decrease fitness (i.e. growth rate). As a consequence, there is a trade-off between the advantages and disadvantages of a mutated receptor, leading to polymorphisms at this locus within bacterial populations coevolving with phages.

Studying host–parasite coevolution

Field studies across space and/or time

Field studies across space at a single time point may be useful to study coevolution for host systems with long generation times, for which temporal changes cannot be easily assessed within the often restricted timeline of research projects. Such spatial studies rely on the concept of local adaptation: Due to chance effects and varying environmental conditions host and parasite are likely to coevolve along different trajectories in different locations. The comparison of locations may reveal various stages of the coevolutionary cycle. A major challenge is to distinguish patterns of local adaptation caused by reciprocal coevolutionary change versus directional selection to any other environmental factor.

Long term field studies of populations from one or more sites represent the ideal approach to analyse the dynamics and consequences of coevolution, because they allow the direct detection of reciprocal genetic change. A particular challenge is to relate the temporal dynamics to variation in environmental factors (i.e. G x G x E effects). Long term studies including several locations may also help to understand the dynamics of the geographic mosaic of coevolution.

Experiments under controlled lab conditions

Laboratory-based evolution experiments may represent a highly efficient approach to dissect the importance of particular selective factors and to characterize in detail the dynamics and consequences of coevolution. Most importantly, they allow to minimize confounding environmental influences and to test single factors, which can be varied between treatments. Therefore, this approach may allow distinction of cause and effect. Such evolution experiments require host systems with short generation times like bacteria or invertebrates. A particular challenge is to relate the results from the artificial laboratory environment to the complexity encountered in nature.

Potamopyrgus antipodarum and its trematode

Potamopyrgus antipodarum

The New Zealand freshwater snail Potamopyrgus antipodarum and its different trematode parasites represent a rather special model system. Populations of P. antipodarum consist of asexual clones and sexual individuals and therefore can be used to study the evolution and advantages of sexual reproduction.

Long term laboratory experiments and field studies revealed that there is a high correlation between the presence of parasites and the frequency of sexual individuals within the different populations. This result is consistent with the Red Queen hypothesis that sexual reproduction is favoured during host–parasite coevolution. At the same time, the persistence of sex may also rely on other factors, for example Muller's ratchet and/or the avoidance of the accumulation of deleterious mutations (deterministic mutation model).

Caenorhabditis elegans and Bacillus thuringiensis

Bacillus thuringiensis

Caenorhabditis elegans

The nematode Caenorhabditis elegans and the bacterium Bacillus thuringiensis were only recently established as a model system for studying host–parasite coevolution. Laboratory evolution experiments provided evidence for many of the basic predictions of these coevolutionary interactions, including reciprocal genetic change, and increases in the rate of evolution and genetic diversity.

The widespread use of C. elegans in different branches of biology makes it a good choice for evolutionary research. On the one hand evolutionary studies may help to close the gaps in knowledge still persistent for C. elegans biology. On the other hand, the information already available on this species may help to work out the molecular mechanisms underlying evolutionary change.

Daphnia and its parasites

Daphnia magna

The crustacean Daphnia and its numerous parasites have become one of the main model systems for studying coevolution. One valuable characteristic of this system is that the reproduction of the host can be asexual as well as sexual (induced by changes in the external environment), so that conditions for sexual reproduction can be stimulated in the laboratory.

Coevolution was especially studied between Daphnia magna and the bacterium Pasteuria ramosa. For example, Decaestecker et al. reconstructed the evolution of the populations across decades, by reanimating resting stages of both species from laminated pond sediments and exposed hosts from each layer to parasites from the past, the same and the future layers. The study demonstrated that parasites were on average most infective on their contemporary hosts in consistency with negative frequency dependent selection

Tribolium castaneum and Nosema whitei

Tribolium castaneum

As many other arthropods Tribolium castaneum, the red flour beetle, is a host for the microsporidia Nosema whitei.

Nosema whitei kills its host for transmission, thus the host’s lifespan is important for the parasite’s success. In turn, parasite fitness most likely depends on a trade-off between transmission (spore load) and virulence: A higher virulence would increase the potential for the production of more offspring, but a higher spore load would affect the host’s lifespan and therefore the transmission rate. This trade-off is supported by coevolutionary experiments, which revealed the decrease of virulence, a constant transmission potential and an increase in the host’s lifespan over a period of time. Further experiments demonstrated a higher recombination rate in the host during coevolutionary interactions, which may be selectively advantageous because it should increase diversity of host genotypes.

Plantago and its parasites

Plantago lanceolata

Host–parasite coevolution is studied in a diversity of plant systems. One example is Plantago lanceolata and its parasite Podosphaera plantaginis, which has been intensively studied on the Aland islands in south-western Finland. P. plantaginis is a powdery mildew obtaining nutrients from its host, a perennial herb, by sending feeding roots into the plant.

There are more than 3000 host populations known in this region, where both populations can evolve freely, in absence of human-imposed selection, in a very heterogeneous landscape. Both antagonists can reproduce asexually or sexually. This system was used to demonstrate spatially divergent coevolutionary dynamics across two metapopulations and it provided support for the predictions of the Mosaic Theory of Coevolution.

Bacteria and their parasites

Biological communities are often complex, they are influenced by diverse factors, and therefore, they are very difficult to study. A possible solution is to focus on simple community systems under controlled laboratory conditions, for example microbial communities consisting of Escherichia coli or Pseudomonas fluorescens and their bacteriophages.

Escherichia coli

E. coli, a Gram-negative proteobacterium, is a common model in biological research, for which comprehensive data on various aspects of its life-history is available. It has been used extensively for evolution experiments, including those related to coevolution with phages. These studies revealed - among others - that coevolutionary adaptation may be influenced by pleiotropic effects of the involved genes. In particular, binding of the bacteriophage to E. coli surface receptor is the crucial step in the virus infection cycle. A mutation in the receptor's binding site may cause resistance. Such mutations often show pleiotropic effects and may cause a cost of resistance. In the presence of phages, such pleiotropy may lead to polymorphisms in the bacterial population and thus enhance biodiversity in the community. Another model system consists of the plant- and animal-colonizing bacterium Pseudomonas and its bacteriophages. This system provided new insights into the dynamics of coevolutionary change. It demonstrated that coevolution may proceed via recurrent selective sweeps, favouring generalists for both antagonists. Furthermore, coevolution with phages may promote allopatric diversity, potentially enhancing biodiversity and possibly speciation. Host-parasite coevolution may also affect the underlying genetics, for example by favouring increased mutation rates in the host.

Links/Further reading

Mosaic Model

Laine AL (2006): Evolution of host resistance: looking for coevolutionary hotspots at small spatial scales. Proceedings of the Royal Society of London: Biological Sciences 273: 267-273 <http://rspb.royalsocietypublishing.org/content/273/1584/267.abstract>

Rudgers JA, Strauss SY (2004): A selection mosaic in the facultative mutualism between ants and wild cotton. Proceedings of the Royal Society of London: Biological Sciences271: 2481-2488

Thompson JN, Cunningham BM (2002): Geographic structure and dynamics of coevolutionary selection. Nature 417:735-738 <http://www.nature.com/nature/journal/v417/n6890/full/nature00810.html>

Thompson JN (1994): The Coevolutionary process.The University of Chicago Press, Chicago

Potamopyrgus antipodarum and its trematodes

Koskella B, Lively CM (2007): Advice of the rose:experimental coevolution of a trematode parasite and its snail host. Evolution61:152-159

EAWAG Department of Aquatic Ecology| EAWAG Department of Aquatic Ecology

Caenorhabditis elegans and Bacillus thuringiensis

Department of Evolutionary Ecology and Genetics, CAU Kiel

Institute for Evolution and Biodiversity, University of Münster

Daphnia and its parasites

TJ Little Lab Homepage

E Decaestecker Lab Homepage

D Ebert Lab Homepage

Duncan AB, Little TJ (2007): Parasite driven genetic change in a natural population of Daphnia. Evolution 61:796-803 <http://www3.interscience.wiley.com/cgi-bin/fulltext/117958810/PDFSTART>

Decaestecker E, Vergote A, Ebert D, De Meester L (2003): Evidence for strong host-clone parasite species interactions in the Daphnia-parasite system. Evolution 57:784-792 <http://www3.interscience.wiley.com/cgi-bin/fulltext/118867883/PDFSTART>

Ebert D (2005). Ecology, Epidemiology, and Evolution of Parasitism in Daphnia. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Books.

Altermatt F, Ebert D (2008): Genetic diversity of Daphnia magna populations enhances resistance to parasites. Ecology letters 11: 918–928 <http://www3.interscience.wiley.com/cgi-bin/fulltext/120125494/PDFSTART>

Tribolium castaneum and Nosema whitei

Experimental Ecology, University of Zürich

Animal Evolutionary Ecology, University Münster

Plantago and its parasites

J Burdon Lab Homepage

I Hanski Lab Homepage

AL Laine Lab Homepage

Burdon J, Thrall P (2009):Coevolution of plants and their pathogens in natural habitats. Science 324,5928:755 - 756 <http://www.scienceonline.org/cgi/content/full/324/5928/755>

Laine A-L, Hanski I (2006): Large-scale spatial dynamics of specialist plant pathogen. Journal of Ecology 92: 990-1000.

Bacteria and their bacteriophages

JN Thompson Lab Homepage

Center of Ecology and Evolutionary Biology, University of Oregon

BJM Bohannan Lab Homepage

Pseudomonas Genome Database