Ohad Knoller is the son of journalist Judith Knoller. He was married until 2011 to actress Noa Raba-Knoller with whom he has a son. Knoller is most notable known for his roles in the Eytan Fox films Yossi and Jagger and The Bubble and as well in the Steven Spielberg movie Munich.
Ohad Knoller attended the Thelma Yellin high school for performing arts in Tel-Aviv. In 1990, when he was 14, he starred in his first TV role on Israeli television. At the age of 18, in 1994, he played a role in the movie Under the Domim Tree with Gila Almagor. After his military service he began to study acting at the Nissan Nativ school of acting in Tel Aviv. His theatre experience at the Jerusalem Khan Theatre included roles such as the starring role in "The Miser". He also appeared in the movie Super Boy (1998). He starred in the hit TV show Srugim as Dr. Nati Brenner from 2008-2012.
- 2012: Urban Tale
- 2012: Yossi
- 2011: Barefoot (TV miniseries)
- 2011: Anachnu Lo Levad
- 2010: Who do you think you are? (TV miniseries)
- 2008: Halakeh
- 2008: Ha'yom Shel Adam
- 2008: Srugim (TV series)
- 2008: Til the Wedding (TV series)
- 2007: Redacted
- 2007: Beaufort
- 2006: The Bubble
- 2005: Munich
- 2005: Bruno's in Love (TV film)
- 2004: Love Hurts
- 2004: Delusions
- 2004: Year Zero 
- 2003: Knafayim (TV mini-series)
- 2002: Yossi & Jagger
- 1995: Under the Domim Tree
In 2003, Knoller won the Tribeca Award as best male actor for his role in Yossi & Jagger.