Pyridoxine 5'-phosphate oxidase

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Pyridoxal 5'-phosphate synthase
File:Pyridoxin 5'-phosphate oxidase2.jpg
EC number
CAS number Template:CAS
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ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

Pyridoxine 5’-phosphate oxidase is an enzyme that catalyzes several reactions in the vitamin B6 metabolism pathway. Pyridoxine-5-P oxidase catalyzes the terminal step (also the rate-limiting step) in vitamin B6 metabolism, the biosynthesis of pyridoxal-5’-phosphate, the biologically active form of vitamin B6 which acts as an essential cofactor.[1] Pyridoxine 5’-phosphate oxidase is a member of the enzyme class oxidases, or more specifically, oxidoreductases. These enzymes catalyze a simultaneous oxidation-reduction reaction. The substrate oxidase enzymes is hydroxlyated by one oxygen atom of molecular oxygen.[2] Concurrently, the other oxygen atom is reduced to water. Even though molecular oxygen is the electron acceptor in these enzymes’ reactions, they are unique because oxygen does not appear in the oxidized product.


Pyridoxine 5’-phosphate oxidase is a homodimer, or a molecule consisting of two identical polypeptide subunits. It is hypothesized that the two monomers are held together via disulfide bonds. There are also salt-bridge interactions between the two monomers. Each subunit tightly binds one molecule of pyridoxal 5'-phosphate tightly on each subunit. Both alpha-helices and beta-sheets are present in the protein motif, which is best described as a split-barrel structure. This structure is due, in part, to the disulfide bonds present in the secondary protein structure of this enzyme. Multiple thiol groups (-SH) indicate the presence of disulfide bonds in the structure of the molecule. This enzyme requires the presence of a cofactor, FMN (flavin mononucleotide).[3] Cofactors are ions or coenzymes necessary for enzyme activity. The FMN is located in a deep cleft (formed by the two polypeptide subunits), and held in place by extensive hydrogen-bond interactions with the protein. In this particular case, the FMN helps the enzyme to bind the substrates. In the absence of pyridoxal 5’-phosphate (PLP), the active site of the enzyme is in an “open” conformation. Once substrate binds and is converted to PLP, the active site of the enzyme is in a partially “closed” conformation. Specific amino acid residues can form hydrogen bonds with the PLP, thus forming a lid that physically covers the active site, giving rise to the “closed” conformation.[4]

File:Pyridoxine 5'-phosphate oxidase.jpg
Pyridoxine 5'-phosphate oxidase structure. PDB: 1G78


Pyridoxine 5’-phosphate oxidase is the enzyme that catalyzes the rate-limited step of the B6 metabolism pathway. Vitamin B6, which is also known as pyridoxine, is a crucial nutrient for the human body, as it is responsible for more bodily functions than any other vitamin. Vitamin B6 is a coenzyme in the metabolism of carbohydrates, fats and proteins. This means that the enzymes which break these entities down for use in the body cannot function unless Vitamin B6 is present to induce a conformational change in the enzyme, thus activating it. Vitamin B6 also plays a role in the synthesis of hormones, red blood cells, neurotransmitters and enzymes. A person who is deficient in Vitamin B6 could suffer from insomnia, as well as suffer damage to the central nervous system.[1]


Pyridoxine 5’-phosphate oxidase catalyzes several reactions; the two most important are the deamination of pyridoxamine-5'-phosphate and the deamination of pyridoxine 5-phosphate, both of which are key intermediates in the metabolism of B6. For a complete map of the B6 metabolism, Pyridoxine 5’-phosphate oxidase’s EC number is[3]

pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2

File:Pyridoxamine reaction.gif

pyridoxine phosphate + oxygen <=> H2O2 + pyridoxal phosphate

File:Pyridoxine reaction.gif

Pyridoxine 5’-phosphate oxidase also plays a role in nitrogen metabolism, converting amines to aldehydes NH3 by the reaction: amine + H2O + oxygen <=> aldehyde + NH3 + H2O2

File:Amine reaction.png


In humans, the pyridoxine 5’-phosphate oxidase enzyme exhibits a low catalytic rate constant of 0.2 sec-1, with low Km values for both pyridoxine 5'phosphate and pyridoxamine 5'-phosphate. The enzyme also has a low turnover rate, meaning that it is relatively slow converting substrate to product. Pyridoxal 5'-phosphate is an effective product inhibitor. Since pyridoxal 5'-phosphate, the active form of vitamin B6, is the product of the metabolic pathway, if it exists in excess, then the pathway need not proceed to keep making product. However, if it exists in low concentrations, then that is a signal for the pathway to synthesize more. This is an example of feedback inhibition.[5]

Pyridoxine 5’-phosphate oxidase in different organisms

Pyridoxine 5’-phosphate oxidase has been highly conserved over time, as there are many similarities between the enzyme as it is found in humans and E. coli. Although there is only 39% retention of amino acid sequence from the E. coli version of the enzyme to the human version, the sequences for the FMN binding site and the substrate active sites are among the very highly conserved portion. One of the key differences is that the human pyridoxine 5’-phosphate oxidase has a higher specificity for the pyridoxamine-5'-phosphate substrate, whereas the pyridoxine 5’-phosphate oxidase in E. coli has a higher specificity pyridoxal-5’-phosphate substrate.[5]


  1. 1.0 1.1 "Vitamin B6". Retrieved 2007-06-03. 
  2. David L. Nelson; Michael M. Cox (2005). Lehninger Principles of Biochemistry, Fourth Edition. New York: W. H. Freeman and Company. ISBN 0-7167-4339-6. 
  3. 3.0 3.1 Online 'Mendelian Inheritance in Man' (OMIM) PYRIDOXAMINE 5-PRIME-PHOSPHATE OXIDASE; PNPO -603287
  4. "RCSB PDB : "Active Site Structure of E. coli pyridoxine 5'-phosphate Oxidase"". Retrieved 2007-06-03. 
  5. 5.0 5.1 Musayev FN, Di Salvo ML, Ko TP, Schirch V, Safo MK (2003). "Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase". Protein Sci. 12 (7): 1455–63. PMC 2323923Freely accessible. PMID 12824491. doi:10.1110/ps.0356203.