MS2 tagging
From Infogalactic: the planetary knowledge core
MS2 tagging is a technique based upon the natural interaction of the MS2 bacteriophage coat protein with a stem-loop structure from the phage genome.[1] Used for biochemical purification of RNA-protein complexes and partnered to GFP for detection of RNA in living cells.[2] More recently, the technique has been used to monitor the appearance of RNA in living cells, at the site of transcription, or simply by observing the changes in RNA number in the cytoplasm.[3][4] This has revealed that transcription of both prokaryotic and eukaryotic genes occurs in a discontinuous fashion (see Transcriptional bursting) with bursts of transcription separated by irregular intervals.
Notes
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- ↑ Bertrand, E., Chartrand, P., Schaefer, M., Shenoy, S. M., Singer, R. H. and Long, R. M. (1998). Localization of ASH1 mRNA particles in living yeast. Mol Cell 2, 437-45.
- ↑ Lua error in package.lua at line 80: module 'strict' not found.
- ↑ Lua error in package.lua at line 80: module 'strict' not found.
