Integrin-linked kinase

Integrin-linked kinase
Rendering based on PDB 2KBX.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2KBX, 3F6Q, 3IXE, 3KMU, 3KMW, 3REP, 4HI8, 4HI9
Identifiers
Symbols	ILK ; HEL-S-28; ILK-1; ILK-2; P59; p59ILK
External IDs	OMIM: 602366 MGI: 1195267 HomoloGene: 3318 ChEMBL: 5247 GeneCards: ILK Gene
EC number	2.7.11.1
Gene ontology Molecular function • protein serine/threonine kinase activity • signal transducer activity • integrin binding • protein binding • ATP binding • SH3 domain binding • protein kinase binding Cellular component • stress fiber • nucleoplasm • cytoplasm • cytosol • plasma membrane • cell-cell junction • focal adhesion • membrane • sarcomere • lamellipodium • cell junction • extracellular matrix • neuronal cell body • costamere • terminal bouton • dendritic shaft • protein complex Biological process • branching involved in ureteric bud morphogenesis • positive regulation of cell-matrix adhesion • outflow tract morphogenesis • protein phosphorylation • negative regulation of protein kinase activity • cell cycle arrest • cell-matrix adhesion • integrin-mediated signaling pathway • cell aging • cell proliferation • positive regulation of cell proliferation • negative regulation of cardiac muscle cell apoptotic process • fibroblast migration • negative regulation of smooth muscle cell migration • peptidyl-serine phosphorylation • nerve development • myelination in peripheral nervous system • positive regulation of cell migration • positive regulation of BMP signaling pathway • myelin assembly • regulation of actin cytoskeleton organization • cell junction assembly • substrate adhesion-dependent cell spreading • positive regulation of phosphorylation • extracellular fibril organization • positive regulation of MAP kinase activity • protein kinase B signaling • negative regulation of neuron apoptotic process • establishment or maintenance of epithelial cell apical/basal polarity • positive regulation of myoblast differentiation • positive regulation of osteoblast differentiation • positive regulation of axon extension • positive regulation of transcription, DNA-templated • negative regulation of smooth muscle cell proliferation • neuron projection morphogenesis • positive regulation of dendrite morphogenesis • protein heterooligomerization • positive regulation of protein kinase B signaling • platelet aggregation • positive regulation of canonical Wnt signaling pathway • negative regulation of neural precursor cell proliferation Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	3611	16202
Ensembl	ENSG00000166333	ENSMUSG00000030890
UniProt	Q13418	O55222
RefSeq (mRNA)	NM_001014794	NM_001161724
RefSeq (protein)	NP_001014794	NP_001155196
Location (UCSC)	Chr 11: 6.6 – 6.61 Mb	Chr 7: 105.74 – 105.74 Mb
PubMed search	[1]	[2]

Integrin-linked kinase (ILK) is a 59kDa protein originally identified while conducting a yeast-two hybrid screen with integrin β1 as the bait protein (Hannigan et al., 1996). Since its discovery, ILK has been associated with multiple cellular functions including cell migration, cell proliferation, cell-adhesions, and signal transduction.

Function

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene was initially described to encode a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. Recent results showed that ILK contains 5 ankyrin-like repeats, and that the C-terminal kinase domain is actually a pseudo-kinase with adaptor function.

In 2008, ILK was found to localize to the centrosome and regulate mitotic spindle organization.

Interactions

Integrin-linked kinase has been shown to interact with:

• ACP6,
• AKT1,
• ILKAP, and
• LIMS1,