S-adenosylmethionine synthetase enzyme
| Methionine adenosyltransferase | |||||||||
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| 270px
S-adenosylmethionine synthase 2, tetramer, Human
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| Identifiers | |||||||||
| EC number | 2.5.1.6 | ||||||||
| CAS number | Template:CAS | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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S-adenosylmethionine synthetase (EC 2.5.1.6) (also known as methionine adenosyltransferase (MAT)) is an enzyme that creates S-adenosylmethionine (AdoMet) by reacting methionine (a non-polar amino acid) and ATP (the basic currency of energy).[1]
Contents
Function
AdoMet is a methyl donor for transmethylation. It gives away its methyl group and is also the propylamino donor in polyamine biosynthesis. S-adenosylmethionine synthetase can be considered the rate-limiting step of the methionine cycle.[2]
S-adenosylmethionine (SAM) is a methyl donor and allows DNA methylation. Once DNA is methylated, it switches the genes off and therefore, S-adenosylmethionine can be considered to control gene expression.[3]
SAM is also involved in gene transcription, cell proliferation, and production of secondary metabolites.[4] Hence SAM synthetase is fast becoming a drug target, in particular for the following diseases: depression, dementia, vacuolar myelopathy, liver injury, migraine, osteoarthritis, and as a potential cancer chemopreventive agent.[5]
This article discusses the protein domains that make up the SAM synthetase enzyme and how these domains contribute to its function. More specifically, this article explores the shared pseudo-3-fold symmetry that makes the domains well-adapted to their functions.[6]
This enzyme catalyses the following chemical reaction
- ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
Conserved motifs in the 3'UTR of MAT2A mRNA
A computational comparative analysis of vertebrate genome sequences have identified a cluster of 6 conserved hairpin motifs in the 3'UTR of the MAT2A messenger RNA (mRNA) transcript.[7] The predicted hairpins (named A-F) have strong evolutionary conservation and 3 of the predicted RNA structures (hairpins A, C and D) have been confirmed by in-line probing analysis. No structural changes were observed for any of the hairpins in the presence of metabolites SAM, S-adenosylhomocysteine or L-Methioninine. They are proposed to be involved in transcript stability and their functionality is currently under investigation.[7]
Protein overview
The S-adenosylmethionine synthetase enzyme is found in almost every organism bar parasites which obtain AdoMet from their host. Isoenzymes are found in bacteria, budding yeast and even in mammalian mitochondria. Most MATs are homo-oligomers and the majority are tetramers. The monomers are organised into three domains formed by nonconsecutive stretches of the sequence, and the subunits interact through a large flat hydrophobic surface to form the dimers.[8]
S-adenosylmethionine synthetase N terminal domain
| S-adenosylmethionine synthetase N terminal domain | |||||||||
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| File:PDB 1mxb EBI.jpg
S-adenosylmethionine synthetase with ADP
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| Identifiers | |||||||||
| Symbol | S-AdoMet_synt_N | ||||||||
| Pfam | PF00438 | ||||||||
| InterPro | IPR022628 | ||||||||
| PROSITE | PDOC00369 | ||||||||
| SCOP | 1mxa | ||||||||
| SUPERFAMILY | 1mxa | ||||||||
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In molecular biology the protein domain S-adenosylmethionine synthetase N terminal domain is found at the N-terminal of the enzyme.
N terminal domain function
The N terminal domain is well conserved across different species. This may be due to its important function in substrate and cation binding. The residues involved in methionine binding are found in the N-terminal domain.[8]
N terminal domain structure
The N terminal region contains two alpha helices and four beta strands.[6]
S-adenosylmethionine synthetase Central domain
| S-adenosylmethionine synthetase Central domain | |||||||||
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| File:PDB 1mxb EBI.jpg
S-adenosylmethionine synthetase with ADP
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| Identifiers | |||||||||
| Symbol | S-AdoMet_synt_M | ||||||||
| Pfam | PF02772 | ||||||||
| InterPro | IPR022629 | ||||||||
| PROSITE | PDOC00369 | ||||||||
| SCOP | 1mxa | ||||||||
| SUPERFAMILY | 1mxa | ||||||||
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Central terminal domain function
The precise function of the central domain has not been fully elucidated, but it is thought to be important in aiding catalysis.
Central domain Structure
The central region contains two alpha helices and four beta strands.[6]
S-adenosylmethionine synthetase, C terminal domain
| S-adenosylmethionine synthetase, C-terminal domain | |||||||||
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| File:PDB 1o92 EBI.jpg
Methionine adenosyltransferase in a complex ADP and l-methionine.
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| Identifiers | |||||||||
| Symbol | S-AdoMet_synt_C | ||||||||
| Pfam | PF02773 | ||||||||
| InterPro | IPR022630 | ||||||||
| PROSITE | PDOC00369 | ||||||||
| SCOP | 1mxa | ||||||||
| SUPERFAMILY | 1mxa | ||||||||
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In molecular biology, the protein domain S-adenosylmethionine synthetase, C-terminal domain refers to the C terminus of the S-adenosylmethionine synthetase
C terminal domain function
The function of the C-terminal domain has been experimentally determined as being important for cytoplasmic localisation. The residues are scattered along the C-terminal domain sequence however once the protein folds, they position themselves closely together.[3]
C terminal domain Structure
The C-terminal domains contains two alpha-helices and four beta-strands.[6]
References
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- ↑ 8.0 8.1 Lua error in package.lua at line 80: module 'strict' not found.
External links
- Methionine adenosyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)
- EC 2.5.1.6
This article incorporates text from the public domain Pfam and InterPro IPR022630
