Ptk2 cells
PtK2 Cells are a cell line derived from male rat-kangaroo (Potorous tridactylis) epithelial kidney cells.[1] This cell line is used for a variety of application in biomedical research but is particularly popular as a model for mitosis.[2]
Origin
The PtK2 cell line was established by Kristen Walen and Spencer Brown in 1962.[3] Cells from the rat kangaroo were selected as the source of a cell line because this species has only a small number of chromosomes and these chromosomes are easily visualized under a microscope. This makes these cells particularly suited to studying mitosis.
Characteristics
PtK2 cells are relatively large and when grown in a monolayer stay flat throughout the cell cycle unlike many cells that round up during mitosis.[4] PtK2 cells are resistant to adenovirus 5, coxsackievirus B5, and poliovirus 2. They are susceptible to coxsackievirus A9, herpes simplex, vaccinia, and vesicular stomatitis (Ogden strain).[5] PtK2 cells contain intermediate filaments composed of Keratin.[6]
References
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