BH3 interacting-domain death agonist

BH3 interacting domain death agonist
250px PDB rendering based on 2bid.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1ZY3, 2BID, 2KBW, 2M5B, 2M5I, 4BD2, 4ZEQ, 5AJJ
Identifiers
Symbols	BID ; FP497
External IDs	OMIM: 601997 MGI: 108093 HomoloGene: 923 ChEMBL: 1250414 GeneCards: BID Gene
Gene ontology Molecular function • death receptor binding • protein binding • ubiquitin protein ligase binding Cellular component • mitochondrion • mitochondrial outer membrane • cytosol • membrane • integral component of mitochondrial membrane • extracellular exosome Biological process • release of cytochrome c from mitochondria • protein targeting to mitochondrion • apoptotic process • activation of cysteine-type endopeptidase activity involved in apoptotic process • brain development • extrinsic apoptotic signaling pathway via death domain receptors • apoptotic mitochondrial changes • programmed cell death • response to estradiol • positive regulation of protein oligomerization • positive regulation of protein homooligomerization • glial cell apoptotic process • regulation of cell proliferation • signal transduction in response to DNA damage • positive regulation of apoptotic process • protein homooligomerization • neuron apoptotic process • establishment of protein localization to membrane • positive regulation of release of cytochrome c from mitochondria • intrinsic apoptotic signaling pathway • hepatocyte apoptotic process • mitochondrial outer membrane permeabilization • positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway • positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway • regulation of G1/S transition of mitotic cell cycle • positive regulation of extrinsic apoptotic signaling pathway • positive regulation of intrinsic apoptotic signaling pathway Sources: Amigo / QuickGO
RNA expression pattern
File:PBB GE BID 204493 at tn.png
File:PBB GE BID 211725 s at tn.png
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	637	12122
Ensembl	ENSG00000015475	ENSMUSG00000004446
UniProt	P55957	P70444
RefSeq (mRNA)	NM_001196	NM_007544
RefSeq (protein)	NP_001187	NP_031570
Location (UCSC)	Chr 22: 17.73 – 17.77 Mb	Chr 6: 120.89 – 120.92 Mb
PubMed search	[1]	[2]

BID
File:PDB 2bid EBI.jpg human pro-apoptotic protein bid
Identifiers
Symbol	BID
Pfam	PF06393
InterPro	IPR010479
SCOP	1ddb
SUPERFAMILY	1ddb
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

The BH3 interacting-domain death agonist, or BID, gene is a pro-apoptotic member of the Bcl-2 protein family. Bcl-2 family members share one or more of the four characteristic domains of homology entitled the Bcl-2 homology (BH) domains (named BH1, BH2, BH3 and BH4), and can form hetero- or homodimers. Bcl-2 proteins act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities.

Interactions

BID is a pro-apoptotic Bcl-2 protein containing only the BH3 domain. In response to apoptotic signaling, BID interacts with another Bcl-2 family protein, Bax, leading to the insertion of Bax into organelle membranes, primarily the outer mitochondrial membrane. Bax is believed to interact with, and induce the opening of the mitochondrial voltage-dependent anion channel, VDAC. Alternatively, growing evidence suggest that activated Bax and/or Bak form an oligomeric pore, MAC in the outer membrane. This results in the release of cytochrome c and other pro-apoptotic factors (such as SMAC/DIABLO) from the mitochondria, often referred to as mitochondrial outer membrane permeabilization, leading to activation of caspases. This defines BID as a direct activator of Bax, a role common to some of the pro-apoptotic Bcl-2 proteins containing only the BH3 domain.

The anti-apoptotic Bcl-2 proteins, including Bcl-2 itself, can bind BID and inhibit BID's ability to activate Bax. As a result, the anti-apoptotic Bcl-2 proteins may inhibit apoptosis by sequestering BID, leading to reduced Bax activation.

The expression of BID is upregulated by the tumor suppressor p53, and BID has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BID. However, p53 also has a transcription-independent role in apoptosis. In particular, p53 interacts with Bax, promoting Bax activation and the insertion of Bax into the mitochondrial membrane.

The BH3 interacting-domain death agonist has been shown to interact with:

• ATR/ATRIP,
• BCL2,
• CASP2,
• CASP8,
• MCL1, and
• RPA.

Cleavage

File:Casp8-BID.gif

Caspase-8 (as surface) cleavage of Bid (as ribbon) (visualization by Kosi Gramatikoff)

Several reports have demonstrated that caspase-8, and its substrate BID, are frequently activated in response to certain apoptotic stimuli in a death receptor-independent manner. N-hydroxy-L-arginine (NOHA), a stable intermediate product formed during the conversion of L-arginine to nitric oxide activates caspase-8. Activation of caspase-8, and subsequent BID cleavage participate in cytochrome-c mediated apoptosis. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mediated activation of caspase-9 via cytochrome-c release has been shown to result in the activation of caspase-8 and Bid cleavage. Aspirin and Curcumin (diferuloylmethane) too activate caspase-8 to cleave and translocated Bid, induced a conformational change in and translocation of Bax and cytochrome-c release.

See also

2